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T84 cells (ATCC, CCL-248) were maintained in HAMs F12/DMEM (1:1) with L-glutamine (ThermoFisher) supplemented with 10% HI-FCS, 25 g/mL streptomycin and 25 U/mL penicillin (ThermoFisher)

With a 15% bulk discount on orders of five or more vials of the same size, five 100mg vials drop from 230,000 to 195,500

Stincone A, Prigione A, Cramer T, Wamelink MM, Campbell K, Cheung E, Olin-Sandoval V, Gruning NM, Kruger A, Tauqeer Alam M, Keller MA, Breitenbach M, Brindle KM, Rabinowitz JD, Ralser M (2015) The return of metabolism: biochemistry and physiology of the pentose phosphate pathway

This combination approach is common in protocols designed to enhance physiologic growth hormone pulses rather than create a blunt or artificial hormonal spike

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